FIG 7.

CAV1 expression regulates occludin expression in the colon. (A) Expression of CAV1 and occludin in Caco-2 and HCT116 cells. (B) Western analysis in Caco-2 cells 24 h following adenovirus-mediated overexpression of CAV1 compared to the results for the control (adenovirus expressing green fluorescent protein). (C) Western analysis in HCT116 cells 48 h following an shRNA-mediated decrease in CAV1 compared to the results for the control (Scramble). Expression of CAV1 and occludin in the colons of Vhl^ΔIE mice treated with MβCD by gavage (D) or intrarectally (E). Veh, vehicle. (F) Occludin gene expression normalized to the level of β-actin gene expression. (G) Membrane occludin and CAV1 expression in the colons of Vhl^ΔIE and littermate control mice (Vhl^flox/flox) treated ex vivo with MG132 (10 μM) or vehicle for 1 h. Four to six mice were assessed per each group. Each bar represents the mean value ± SD. N.S., not significant.