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Impaired UB outgrowth in Wnt5a-deficient kidneys is associated with the loss of proper interaction between the MM and UB. (A to E) Kidneys expressing HoxB7-EGFP (green) were isolated from embryos at E11.5 with the indicated genotypes. (A′ to E′) After organ culture for 72 h, the kidneys were subjected to whole-mount immunofluorescence staining with anti-Pax2 antibody (red). Magnified images of the boxed regions are shown at the bottom. The arrows indicate MesTs. Scale bars, 0.1 mm.
