Skip to main content
. 2014 Aug;34(16):3120–3131. doi: 10.1128/MCB.00623-14

FIG 7.

FIG 7

Tendon differentiation is compromised in the craniofacial region of Wnt1-Cre; Alk5fl/fl mice. (A to F) In situ hybridization of Scleraxis in the tongue (A and B), eye (C and D), and masticatory (E and F) regions of E13.5 control and Wnt1-Cre; Alk5fl/fl mice. The arrows indicate expression of Scleraxis in control mice. (G) Real-time RT-PCR of Scleraxis in the tongues of E11.5, E12.5, and E13.5 control and Wnt1-Cre; Alk5fl/fl (mutant) mice. Scale bars, 200 μm. (H to J) The Alk5-mediated TGF-β signaling pathway in CNCCs contributes to early and late myogenesis in the craniofacial region. Bmp4 from CNCCs induces proliferation of myogenic precursors during early myogenesis at E11.5 and E12.5. Later, TGF-β signaling in CNCCs induces the expression of Fgf4 and Fgf6 in myogenic cells, which is critical for myogenic differentiation of the craniofacial muscles. NT, neural tube; Md, mandible; GF, growth factors. The error bars indicate standard deviation.