FIG 4.

HGF stimulates Rac1-dependent association of IQGAP1 with the MT fraction. HPAEC were stimulated with HGF (50 ng/ml); this was followed by isolation of the MT-enriched fractionation. Western blotting was used to detect IQGAP1 in the MT fraction, normalized to tubulin content, and in total lysates isolated from control and HGF-treated EC. (A) Time course analysis of HGF-induced IQGAP1 association with the MT fraction. (B) Effect of c-Met inhibitor (inh; 50 nM carboxamide, 30 min) on HGF-induced IQGAP1 association with the MT fraction. (C) Effect of siRNA-induced Rac1 knockdown (50 nM, 48 h) on HGF-induced IQGAP1 association with the MT fraction. Rac1 protein depletion was confirmed by Western blot analysis of total cell lysates with a Rac1 antibody. Results are representative of three to five independent experiments.