FIG 8.
Decreased survival of DP thymocytes in ERK3-deficient mice. (A) mRNA quantification of the antiapoptotic Mcl1 and proapoptotic Pmaip1 genes in sorted DP thymocytes from Erk3+/+ and Erk3−/− mice. (B) mRNA quantification of the Bcl2l11, Bcl2a1, and Rorc genes in sorted DP thymocytes from Erk3+/+ and Erk3−/− mice. (C) Bcl-xL representative overlay histogram gated on DP thymocytes from Erk3+/+ and Erk3−/− mice. Fluorescence minus one (FMO) was used as a negative control. (D) Quantification of DP thymocyte cell death. Annexin V-positive (AnnV+) cells among total, TCRlo, TCRint, and TCRhi DP thymocyte populations after 5 days of culture in FTOCs are shown. Data are representative of at least 2 independent experiments. *, P < 0.05, two-tailed Student's t test.