TABLE 3.

Primers for measurement of TCRα secondary gene rearrangement by semiquantitative RT-PCR

Target	Primer sequence
	Forward (5′–3′)	Reverse (5′–3′)
Vα3-Cα	CCCAGTGGTTCAAGGAGTGA	TTCAGCAGGAGGATTCGGAG
Vα6-Cα	CTGACTCATGTCAGCCTGAGAG	TTCAGCAGGAGGATTCGGAG
Vα8-Cα	CAACAAGAGGACCGAGCACC	TTCAGCAGGAGGATTCGGAG
Vα14-Cα	TGGGAGATACTCAGCAACTCTGG	TTCAGCAGGAGGATTCGGAG
Vα19-Cα	CTGCTTCTGACAGAGCTCCAG	TTCAGCAGGAGGATTCGGAG
Vα3-Jα2	CCCAGTGGTTCAAGGAGTGA	ACCACTTAGTCCTCCAGTATTC
Vα3-Jα18	CCCAGTGGTTCAAGGAGTGA	CAGGTATGACAATCAGCTGAGTCC
Vα3-Jα30	CCCAGTGGTTCAAGGAGTGA	AGATGTGTCCCTTTTCCAAAGATG
Tcra constant region (Cα)	TTCAAAGAGACCAACGCCAC	TTCAGCAGGAGGATTCGGAG