Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers for quantification of TCRα secondary gene rearrangement by qPCR
| Target | Primer sequence |
|
|---|---|---|
| Forward (5′–3′) | Reverse (5′–3′) | |
| Vα3-Jα30 | CCCAGTGGTTCAAGGAGTGA | AGATGTGTCCCTTTTCCAAAGATG |
| Vα6-Cα | ACCACTGTATCCTGAGAGTAG | CTGGTACACAGCAGGTTC |
| Vα19-Cα | CTGCTTCTGACAGAGCTCCAG | CTGGTACACAGCAGGTTC |
| Vα3-Jα2 | CCCAGTGGTTCAAGGAGTGA | ACCACTTAGTCCTCCAGTATTC |
| Tcra constant region (Cα) | TTCAAAGAGACCAACGCCAC | TTCAGCAGGAGGATTCGGAG |
