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. 2014 Sep;34(18):3500–3514. doi: 10.1128/MCB.00519-14

FIG 2.

FIG 2

Nts1, Mug165, Png3, and Fkh2 are integral components of the Clr6 HDAC. (A and B) TAP-tagged proteins were captured on IgG-Sepharose from whole-cell lysates of strains producing the indicated TAP- and Myc-tagged proteins and analyzed by peroxidase-antiperoxidase and anti-Myc Western blotting. Input samples were 1% of the total protein used in the pulldown. For the pulldown samples, we loaded 20% (or 25%) (vol/vol) of the IgG resin eluate.