FIG 5.

Nts1 localizes to SPNCRNA.276 independently of its normal genomic context. (A) Schematic representation of the locus encompassing the α-glucosidase (gto1), SPNCRNA.1087, SPNCRNA.276, and SPNCRNA.30 genes. The position at which Nts1 localizes within this region is shown as a ChIP-seq peak. The regions that were “transposed” to the leu1 locus are shown and labeled as pNZ84, pNZ86, and pNZ87. Chromosome coordinates are expressed in bp. (B) Nts1 localizes to an ectopic SPNCRNA.276 locus. Anti-FLAG ChIP assays were carried out as described in Fig. 4E. (C) Sds3 copurifies with DNA from Tf2 LTRs and SPNCRNA.276 in an nts1- and mug165-dependent manner. Anti-FLAG ChIP assays were carried out as described for Fig. 4E.