FIG 6.

(A) Deletion of nts1 leads to an increase in H3K9ac levels at the aes1 promoter. Anti-H3K9ac ChIP assays were carried out on the indicated strains and analyzed by qPCR with primer sets annealing to the dg repeat found within the otr, a locus found approximately 486 bp upstream of aes1's start codon (aes1 promoter) and SPNCRNA.276. (B) Clr6 copurifies with DNA from SPNCRNA.276. ChIP assays were carried out on the indicated strains and analyzed by qPCR with primer sets annealing to SPNCRNA.276 and act1. (C) Schematic representation of complexes I (core subunits, Clr6L, analogous to Rpd3L in budding yeast), I′, I′′, and II (Clr6S, analogous to Rpd3S in budding yeast).