TABLE 2.
List of proteins that were detected by mass spectrometry in a pulldown of Nts1a
| Protein | Gene ID | NSAFe5 scored |
Rank no. | |
|---|---|---|---|---|
| FLAG3-Nts1-TAP PD | Control PD | |||
| Nts1b | SPCC24B10.19c | 5211 | 0 | 8 |
| Sds3c | SPAC25B8.02 | 2942 | 0 | 13 |
| Prw1c | SPAC29A4.18 | 2319 | 0 | 18 |
| Clr6c | SPBC36.05c | 1916 | 0 | 21 |
| Mug165c | SPAC5D6.02c | 1747 | 0 | 22 |
| Png3c | SPAC16E8.12c | 1628 | 0 | 24 |
| PstIc | SPBC12C2.10c | 1020 | 0 | 47 |
| Pst3c | SPBC1734.16c | 674 | 0 | 65 |
| Fkh2c | SPBC16G5.15c | 631 | 0 | 69 |
| Rtx3c | SPCC1259.07 | 577 | 0 | 71 |
| Histone H2B | SPCC622.09 | 399 | 0 | 94 |
| Laf2c | SPCC1682.13 | 324 | 0 | 98 |
| Rxt2c | SPBC428.06c | 300 | 0 | 100 |
| Histone H4 | Multiple | 163 | 0 | 120 |
| Laf1c | SPAC14C4.12c | 136 | 0 | 126 |
| Rad25 | SPAC17A2.13c | 129 | 0 | 127 |
| Rad24 | SPAC8E11.02c | 129 | 0 | 128 |
| Nop56 | SPBC646.10c | 93 | 0 | 133 |
| Mlo3 | SPBC1D7.04 | 77 | 0 | 137 |
| Histone H2A | Multiple | 74 | 0 | 138 |
| HMG box protein | SPBC28F2.11 | 63 | 0 | 142 |
| Rvb1 | SPAPB8E5.09 | 61 | 0 | 144 |
| Rpt4 | SPCC1682.16 | 58 | 0 | 146 |
| Tcg1 | SPBC660.11 | 32 | 0 | 156 |
| Rpt3 | SPCC576.10c | 29 | 0 | 161 |
| Meu27 | SPCC1259.14c | 28 | 0 | 162 |
| Rpt6 | SPBC23G7.12c | 28 | 0 | 163 |
| Predicted ubiquitin ligase subunit | SPBC106.13 | 28 | 0 | 164 |
| Nop58 | SPAC23G3.06 | 28 | 0 | 165 |
| Rpt5 | SPAC3A11.12c | 26 | 0 | 169 |
| Oga1 | SPBC16A3.08c | 25 | 0 | 172 |
| Rsc58 | SPAC1F3.07c | 24 | 0 | 173 |
| Predicted human LYAR homologue | SPBC215.06c | 24 | 0 | 177 |
| Cpc2 | SPAC6B12.15 | 22 | 0 | 181 |
| Ump1 | SPCC14G10.03c | 22 | 0 | 184 |
| Ssr3 | SPAC23G3.10c | 16 | 0 | 199 |
| Rvb2 | SPBC83.08 | 15 | 0 | 205 |
| But2 | SPBC3D6.02 | 14 | 0 | 209 |
| Rpn11 | SPAC31G5.13 | 9 | 0 | 232 |
| Tci1 | SPBC16D10.01c | 8 | 0 | 235 |
| Tubulin (β subunit) | SPBC26H8.07c | 6 | 0 | 241 |
| Tubulin (α subunit) | Multiple | 6 | 0 | 242 |
| Rsc4 | SPBC1734.15 | 5 | 0 | 249 |
| Cdc22 | SPAC1F7.05 | 3 | 0 | 258 |
| Cdc48 | SPAC1565.08 | 3 | 0 | 259 |
a
Nts1 interacts with several components of the Clr6 HDAC.
b
Target protein subjected to affinity purification.
c
Top hits that are known components of the Clr6 HDAC.
d
PD, pulldown. NSAFe5 is a semiquantitative assessment of protein abundance.