FIG 2.

Rim8 and Rim13 accumulate at the plasma membrane in yeast cells exposed to alkaline medium. (A) YOK3410 (RIM8-2 × EGFP) and YOK3411 (RIM8-2 × EGFP rim21Δ) cells were subjected to fluorescence microscopy after exposure to alkaline medium for the indicated time. Bar, 5 μm. (B) The percentage of the cells with clear Rim8 foci at the plasma membrane is presented. Values are the means ± SD of the results of three independent experiments (**, P < 0.01; Student's t test). (C) YOK3271 (RIM13-2 × EGFP) and YOK3272 (RIM13-2 × EGFP rim21Δ) cells were subjected to fluorescence microscopy after exposure to alkaline medium for the indicated time. Bar, 5 μm.