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. 2014 Sep;34(18):3525–3534. doi: 10.1128/MCB.00408-14

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FIG 4 — Endocytosis is not essential for the Rim101 pathway. (A) Total lysates were prepared from BY4741 (WT), 1150 (rim21Δ), and MYY312 (end3Δ) cells harboring pFI1 (HA-RIM101) before and 20 min after external alkalization. Immunoblotting was performed with an anti-HA antibody. Two independent end3Δ clones (#1 and #2) were analyzed. FL and ΔC, full-length and processed Rim101, respectively. (B) YOK3073 (WT), YOK3094 (rim21Δ), and YOK3159 (LAS17–HA-AID) cells in log phase were pretreated with 500 μM IAA or ethanol (mock) for 1 h and exposed to alkaline medium for 20 min. Total cell lysates were subjected to immunoblotting with an anti-HA antibody. FL and ΔC, full-length and processed Rim101, respectively.