TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| SEY6210 | MATα his3 leu2 ura3 trp1 lys2 suc2 | 28 |
| YOK2027 | SEY6210 rim21Δ::KanMX4 | 11 |
| YOK3248 | SEY6210 RIM20-EGFP::TRP1 | This study |
| YOK3249 | SEY6210 RIM20-EGFP::TRP1 rim21Δ::KanMX4 | This study |
| YOK3265 | SEY6210 RIM20-EGFP::TRP1 lem3Δ::KanMX4 | This study |
| YOK3334 | SEY6210 RIM20-EGFP::TRP1 PMA1-mCherry::KanMX6 | This study |
| YOK3306 | SEY6210 RIM20-EGFP::TRP1 rim8Δ::KanMX4 | This study |
| YOK3266 | SEY6210 RIM20-EGFP::TRP1 vps4Δ::URA3 | This study |
| YOK3410 | SEY6210 RIM8-2×EGFP::KanMX6 | This study |
| YOK3411 | SEY6210 RIM8-2×EGFP::KanMX6 rim21Δ::NatNT2 | This study |
| YOK3271 | SEY6210 RIM13-2×EGFP::KanMX6 | This study |
| YOK3272 | SEY6210 RIM13-2×EGFP::KanMX6 rim21Δ::NatNT2 | This study |
| YOK3250 | SEY6210 SNF7-mRFP::NatNT2 | This study |
| YOK3251 | SEY6210 SNF7-mRFP::NatNT2 rim21Δ::KanMX4 | This study |
| YOK3598 | SEY6210 SNF7-mRFP::NatNT2 vps4Δ::HIS3 | This study |
| YOK3408 | SEY6210 SNF7-mRFP::NatNT2 RIM20-EGFP::TRP1 | This study |
| YOK3527 | SEY6210 RIM20-mCherry::NatNT2 RIM8-2×EGFP::KanMX6 | This study |
| YOK3528 | SEY6210 RIM20-mCherry::NatNT2 RIM13-2×EGFP::KanMX6 | This study |
| YOK3418 | SEY6210 SNF7-mRFP::NatNT2 RIM20-EGFP::TRP1 vps27Δ::KanMX4 | This study |
| YOK2279 | SEY6210 RIM8-HA::KanMX6 | This study |
| YOK2649 | SEY6210 RIM8-HA::NatNT2 | This study |
| YOK2658 | SEY6210 RIM8-HA::NatNT2 doa4Δ::KanMX4 | This study |
| YOK2562 | SEY6210 rim8Δ::KanMX4 | This study |
| YOK3073 | SEY6210 PADH-OsTIR1-myc::URA3 | This study |
| YOK3094 | SEY6210 PADH-OsTIR1-myc::URA3 rim21Δ::KanMX | This study |
| YOK3159 | SEY6210 PADH-OsTIR1-myc::URA3 LAS17-HA-AID::KanMX6 | This study |
| YOK3393 | SEY6210 PADH-OsTIR1-myc::URA3 PADH-AID-RSP5::NatNT2 | This study |
| YOK3403 | SEY6210 PADH-OsTIR1-myc::URA3 PADH-AID-RSP5::NatNT2 RIM20-EGFP::TRP1 | This study |
| YOK3412 | SEY6210 PADH-OsTIR1-myc::URA3 RIM8-2×EGFP::KanMX6 | This study |
| YOK3413 | SEY6210 PADH-OsTIR1-myc::URA3 PADH-AID-RSP5::NatNT2 RIM8-2×EGFP::KanMX6 | This study |
| YOK3384 | SEY6210 RIM21-2× EGFP::KanMX6 rim8Δ::NatNT2 | This study |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | 36 |
| YOK3427 | BY4741 RIM20-EGFP::KanMX6 | This study |
| YOK3428 | BY4741 RIM8-2× EGFP::KanMX6 | This study |
| YOK3429 | BY4741 RIM13-2× EGFP::KanMX6 | This study |
| 1150 | BY4741 rim21Δ::KanMX4 | 37 |
| 5381 | BY4741 vps27Δ::KanMX4 | 37 |
| MYY312 | BY4741 end3Δ::KanMX4 | This study |
| YPH499 | MATa his3Δ200 lys2-801 ade2-101 leu2Δ1ura3-52 trp1Δ63 | 23 |
| FAY51R | YPH499 rsp5-1 | 33 |
| YOK3342 | YPH499 RIM20-EGFP::TRP1 | This study |
| YOK3343 | YPH499 rsp5-1 RIM20-EGFP::TRP1 | This study |