FIG 6.
Irreversible attachment of class II Ctp mutants: Coverslips were incubated with cell cultures for 1 h, subjected to robust rinsing, stained with af-WGA for 30 min, and then vigorously rinsed again. The total numbers of attached cells per field of view (∼3.13 × 104 μm2) were determined with a Nikon E800 at ×100 magnification in bright-field mode. Enumeration of UPPs was done by determining the number of af-WGA-stained red fluorescent dots by fluorescence microscopy. The values are mean results of 10 fields of view, and the error bars show the standard deviations.