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Trafficking of BAB-parental, BABΔppdK, and the virB mutant in macrophages and HeLa cells. (A) Confocal images of infected RAW 264.7 macrophages and HeLa cells labeled with monoclonal antibodies to either calnexin (green) or LAMP-1 (blue) 24 h after infection (bacteria are immunostained in red). (B) Percentage of calnexin-positive vacuoles of HeLa cells that contain bacteria at 24 and 48 h postinfection.
