TABLE 3.
Bacterial two-hybrid analysis of TraK interactions
| Bait plasmid | Target plasmid | CFU/ml ona: |
|
|---|---|---|---|
| Nonselective plates | Selective plates | ||
| pBT (empty vector) | pKH87 (TraKR53-G244) | 4,164 ± 879 | 0 |
| pKH86 (TraVS20-R193) | pTRG (empty vector) | 48 ± 11 | 0 |
| pKH86 (TraVS20-R193) | pKH87 (TraKR53-G244) | 953 ± 501 | 887 ± 391 |
| pKH103 (TraDM1-V680) | pKH87 (TraKR53-G244) | 966 ± 98 | 0 |
| pKH103 (TraDM1-V680) | pKH170 (TraVS20-R193) | 9,471 ± 2,316 | 0 |
a
The average ± standard deviation of four independent experiments is reported. Nonselective plates contained minimal medium plus 25 μg/ml chloramphenicol (Cam) and 12.5 μg/ml tetracycline (Tet). Selective plates contained minimal medium plus 25 μg/ml Cam, 12.5 μg/ml Tet, and 5 mM 3-AT.