FIG 6.

B. anthracis S-layer assembly with and without UDP-GlcNAc 2-epimerase expression. Spores derived from B. anthracis Sterne(pJK4) and YW6(pJK4) were diluted into BHI without (−IPTG) or with (+IPTG) 1 mM IPTG supplement and incubated for 3 h. Differential interference contrast (DIC) and fluorescence microscopy images were acquired from bacilli stained with BODIPY-vancomycin (B-vanc) or with rabbit antibodies against S-layer proteins Sap (α-Sap) (A), EA1 (α-EA1) (B), or BslR (α-BslR) and secondary antibody conjugates to Alexa Fluor 594. Bars, 5 μm. Data are representative of three independent experimental determinations.