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. 2014 Aug;196(16):3023–3035. doi: 10.1128/JB.01782-14

FIG 2.

FIG 2

The plasmid-borne dinB+ mediates survival loss only in dnaE915 strains. (A) The dnaE+ ΔdinB strain with the plasmid-borne dinB+ (pdinB+) is resistant to MMS (7.5 mM) or NFZ (7.5 μM) compared to the strain carrying the vector. The dnaE915 ΔdinB/pdinB+ strain is more resistant to NFZ than cells carrying the vector; however, survival loss is evident in the ΔdinB dnaE915 strain carrying the plasmid-borne pdinB+ or the catalytically inactive pdinB(D103N) allele upon MMS treatment (7.5 mM). (B) Plasmid-borne pdinB+ does not affect survival in the dnaE+ ΔdinB upon UV treatment. UV irradiation (∼40 J/m2) results in survival loss in the dnaE915 ΔdinB strain carrying pdinB+ or pdinB(D103N). x, <0.001% survival. The percent survival is calculated based on the CFU/ml values for the untreated cells, which are similar for all strains (∼1 × 109 CFU/ml) except the dnaE915 ΔdinB/pdinB(D103N) strain, which has ∼1 × 108 CFU/ml. Error bars represent the standard deviations of the means from the analysis of at least 3 independent isolates.