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. 2014 Sep;196(17):3122–3133. doi: 10.1128/JB.01698-14

TABLE 1.

Acceptor substrate specificities of WbwCECO104 and WbwCECO5a

Compound Concn(mM) Relative activity
WbwCECO104 WbwCECO5
GalNAcα-PO3-PO3-(CH2)11-O-Ph (compound 8) 0.1 100 100
GalNAcα-PO3-PO3-AnthrUb 0.1 49.0 46.1
GlcNAcα-PO3-PO3-(CH2)11-O-Ph (compound 14) 1 <1 <1
GalNAcα-Bn 1 <1 <1
4-Deoxy-GalNAcα-Bn 1 <1 <1
A-(GalNAcα)T 1 <1 <1
N-Ac-V-(GalNAcα)TP-NH2 1 <1 <1
GalNAc 1 <1 <1
AHGVT-(GalNAcα)SAPDTRPAPGSTAPPA 1 <1 <1
GalNAcβ1–4GlcNAcβ-Bn 1 <1 <1
a

Neutral compounds were assayed by the AG1 × 8 method; compounds 8 and 14 and GalNAcα-PO3-PO3-AnthrU were assayed by the C18 Sep-Pak method with 0.435 mM UDP-Gal in the assay (23, 28, 29).

b

The compound solution contained other minor components.