FIG 3.

Phosphorimages of gel mobility shift assays illustrating the DNA binding function of the RegA mutant proteins analyzed as described in the Fig. 2 legend. ³²P-labeled probe-1234 was used to bind RegA. RegA mutant proteins were assessed relative to RegA-wt for DNA binding and oligomerization as a function of the RegA concentration. (A) RegAP57A and RegAE67K. (B) RegAT163P and RegAT173P. (C) RegAA164V and RegAL167P. (D) RegAL174W and L174A. For the complete list of all RegA mutants and their binding of probe-1234 DNA, see Tables 2 and 3.