TABLE 2.
RegA protein | CbbR interaction (probe-0)a | DNA binding |
|
---|---|---|---|
Probe-1234b | Lowest [RegA] (nM)c | ||
wt | + | +++ | 40 |
Receiver domain | |||
D20A/D21A | − | + | 160 |
R27L | + | ++ | 40 |
M32K | − | − | No binding |
R35L | + | ++ | 40 |
K49E | − | ++ | 40 |
P57A | − | ++ | 40 |
D63A(-P) | + | +++ | 40 |
E67K | + | ++ | 40 |
R79G | − | + | 40 |
D84A | + | +++ | 40 |
T91A | + | +++ | 40 |
A97S | + | +++ | 40 |
D109A | + | +++ | 40 |
K113M | + | +++ | 40 |
Linker | |||
P134A | + | +++ | 40 |
For CbbR interaction, + denotes interaction and − denotes no interaction.
For DNA binding, +++ denotes RegA/promoter complex size comparable to wild-type RegA size, ++ denotes complex size reduced relative to wild-type RegA size, + denotes severe reduction of complex size relative to wild-type RegA size, and − denotes no DNA binding relative to wild-type RegA binding.
Lowest concentration of RegA that bound probe-1234.