FIG 4.

In vivo analysis of developmental defects for cheY7 mutants. cheY7 mutant alleles predicted to affect phosphorylation (D53A) or interaction with downstream targets (F107A) were assayed for defects in development. Cells were plated on CFL starvation agar and assessed for aggregation. Each allele is under the control of a constitutive pilA promoter and was integrated at the Mx8 phage attachment site. The vector control represents an insertion at the ectopic locus without a corresponding cheY allele.