TABLE 2.
Results of bacterial two-hybrid analysis
| Protein coupled to plasmid |
β-Galactosidase activity/μg of protein (mean ± SD) | % of WT | |
|---|---|---|---|
| pUT18c | pKT25 | ||
| 0.53 ± 0.05 | |||
| CheY7 | 0.43 ± 0.09 | ||
| CheA7 | 0.54 ± 0.05 | ||
| CheY7 | CheA7 | 29.93 ± 1.82 | 100.0 |
| Cpc7 | 0.65 ± 0.08 | ||
| CheY7 | Cpc7 | 15.21 ± 1.01 | 100.0 |
| D53A | Cpc7 | 5.26 ± 3.17 | 34.6 |
| D53E | Cpc7 | 7.40 ± 2.49 | 48.7 |
| G86A | Cpc7 | 4.25 ± 0.11 | 28.0 |
| R91A | Cpc7 | 13.35 ± 1.86 | 87.8 |
| T92A | Cpc7 | 17.23 ± 4.81 | 113.3 |
| R93A | Cpc7 | 10.65 ± 1.94 | 70.0 |
| F102A | Cpc7 | 13.19 ± 1.82 | 86.7 |
| M103A | Cpc7 | 12.74 ± 1.77 | 83.8 |
| T104A | Cpc7 | 5.73 ± 3.75 | 37.7 |
| P106A | Cpc7 | 0.65 ± 0.25 | 4.3 |
| F107A | Cpc7 | 0.87 ± 0.64 | 5.7 |
| E115A | Cpc7 | 8.12 ± 0.66 | 53.4 |