FIG 1.

Genetic linkage of cnm and pgfS in S. mutans. (A) In all Cnm⁺ strains, cnm is located within a unique 5-kbp region between the core genes SMU.2067 and SMU.2069. Two adjacent genes (cnaB and cbpA) present in all cnm⁺ strains were described previously (15). SMU.2067 (pgfS) encodes a putative glycosyltransferase, whereas SMU.2066c encodes an integral membrane protein with homology to yfhO of B. subtilis. A summary of semiquantitative RT-PCR analysis revealed the cotranscription of cnm and pgfS. Putative Rho-independent transcriptional terminators are indicated with lollypops. (B) Conserved predicted domains of Cnm and PgfS. SS, secretion signal; A, collagen-binding domain; B, threonine-rich repeat domain; LPXTG, cell wall anchor motif; GT, DPM1-like glycosyltransferase domain; TM, transmembrane domain.