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WGA and sWGA lectin analysis of Cnm. Detection and identification of carbohydrates attached to Cnm in S. mutans strains using biotinylated lectins is shown. Whole-cell lysates were separated by SDS-PAGE, transferred onto PVDF membranes, and probed with 20 μg ml−1 WGA (A) or 20 μg ml−1 sWGA (C). (B) Purified Cnm was treated with sialidase for 20 h and probed with WGA. (D) WGA detection of purified Cnm was blocked using increasing concentrations of N-acetylglucosamine.
