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Translation from both from ATG-1 and ATG-2 is needed for optimal expression of the agnK12 gene transcriptional fusions. (A) Effects of inactivation of ATG-1 and ATG-2, or of termination of translation soon after its initiation, on agnK12 gene expression. (B) Analysis of the stability of the lacZ mRNA in MV203 (agnK12′-lacZ) (●) and MV747 (agnK12′-lacZ, CTG-1) (■) by using qRT-PCR. See Materials and Methods for additional details.
