TABLE 1.

E. coli strain MC4100 derivates used to determine gene expression with β-galactosidase as a reporter^a

Strain	Region (nt) of agnK12 in agnK12′-lacZ λRS lysogen	Reference
Transcriptional fusions
MV203	−466–+275	9
MV312	−40–+275	11
MV505	−466–+62	11
MV557	−40–+62	11
MV631	−40–+275, Δ+63–+160	This study
MV654	−466–+88	This study
MV747	−466–+275, +67A→+67C resulting in CTG	This study
MV781	−466–+275, Δ+161–+195	This study
MV812	−466–+275, Δ+161–+195, +67A→+67C	This study
MV846	−466–+275, +223A→+223C, resulting in CTG	This study
MV897	−466–+88, +67A→+67C, resulting in CTG	This study
MV915	−466–+275, +78C→+78A resulting in TAA	This study
MV928	−466–+275, +67A→+67C, +223A→+223C	This study
MV977	−466–+275, +162 GCCGGT +167→+162 CGGCCA +167, +185 ACCGGC +190→+185 TGGCCG +190	This study
Translational fusions
MV689	−466–+125	This study
MV690	−466–+275	This study
MV721	−466–+275, +67A→+67C resulting in CTG-1	This study
MV749	−466–+275, Δ+79–+117, in-frame deletion of 13 amino acids	This study
MV751	−466–+275, Δ+79–+116, out-of-frame deletion of 13 amino acids	This study
MV766	−466–+275, Δ+79–+147, in-frame deletion of 23 amino acids	This study

^aAll strains contain the Dam-encoding plasmid pTP166, and therefore, expression of agn^K12 is locked in the ON phase (11).