Escherichia coli |
|
|
Top-10 |
recA1 araD139 Δ(ara-leu)7697
|
Life Technologies |
SMC117 |
S17-1(λpir); thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7
|
65 |
SMC31 |
DH5α; supE44 lacU169(80lacZM15) hsdR17 thi-1 relA1 recA1
|
66 |
Pseudomonas fluorescens |
|
|
SMC6370 |
Pseudomonas fluorescens Pf0-1; wild type |
67 |
SMC4798 |
P. fluorescens Pf0-1 expressing LapA-HA, inserted after residue 4093; designated the wild type for this study |
30 |
SMC5207 |
ΔlapG; SMC4798 with an unmarked deletion of lapG
|
27 |
SMC5152 |
ΔlapD; SMC4798 with an unmarked deletion of lapD
|
28 |
SMC6414 |
Δhelix; SMC4798 with an unmarked deletion of lapA codons A81–95A |
This study |
SMC6458 |
Δhelix lapG::pKO3-Tetr; SMC4798 with an unmarked deletion of LapA amino acids A81–95A and single-crossover knockout mutation disrupting the lapG gene |
This study |
SMC5847 |
ΔRpts; SMC4798 with an unmarked deletion of LapA amino acids D273–3982S |
This study |
SMC5849 |
ΔCTerm; SMC4798 with an unmarked deletion of LapA amino acids T4018–5151N |
This study |
SMC5853 |
ΔvWA; SMC4798 with an unmarked deletion of LapA amino acids N4736–4963S |
This study |
SMC5851 |
ΔRTX; SMC4798 with an unmarked deletion of LapA amino acids G5020–5144W |
This study |
SMC5855 |
ΔT1SS; SMC4798 with an unmarked deletion of LapA amino acids N5151–5218S |
This study |
SMC5917 |
lapA AA108–109RR; SMC4798 with point mutation AA108–109RR |
27 |
SMC5915 |
Pf0-1 lapG::pKO3-Tetr; SMC4798 with a single-crossover knockout mutation disrupting the lapG gene |
27 |
SMC5927 |
lapA A108W; SMC4798 with point mutation A108D |
This study |
SMC5923 |
lapA AA108–109DD; SMC4798 with point mutation AA108–109DD |
This study |
SMC5925 |
lapA A109D; SMC4798 with point mutation A109D |
This study |
SMC6400 |
Pf0-1 lapG::pKO3-Tetr; SMC6370 with a single-crossover knockout mutation disrupting the lapA gene |
30 |
SMC5869 |
lapA ΔA108–120A; SMC4798 with an unmarked deletion of lapD and an unmarked deletion of LapA amino acids A108–120A |
This study |