TABLE 1.
Strains used in this study
| Organism and strain | Genotype and/or description | Source or reference |
|---|---|---|
| Escherichia coli | ||
| Top-10 | recA1 araD139 Δ(ara-leu)7697 | Life Technologies |
| SMC117 | S17-1(λpir); thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7 | 65 |
| SMC31 | DH5α; supE44 lacU169(80lacZM15) hsdR17 thi-1 relA1 recA1 | 66 |
| Pseudomonas fluorescens | ||
| SMC6370 | Pseudomonas fluorescens Pf0-1; wild type | 67 |
| SMC4798 | P. fluorescens Pf0-1 expressing LapA-HA, inserted after residue 4093; designated the wild type for this study | 30 |
| SMC5207 | ΔlapG; SMC4798 with an unmarked deletion of lapG | 27 |
| SMC5152 | ΔlapD; SMC4798 with an unmarked deletion of lapD | 28 |
| SMC6414 | Δhelix; SMC4798 with an unmarked deletion of lapA codons A81–95A | This study |
| SMC6458 | Δhelix lapG::pKO3-Tetr; SMC4798 with an unmarked deletion of LapA amino acids A81–95A and single-crossover knockout mutation disrupting the lapG gene | This study |
| SMC5847 | ΔRpts; SMC4798 with an unmarked deletion of LapA amino acids D273–3982S | This study |
| SMC5849 | ΔCTerm; SMC4798 with an unmarked deletion of LapA amino acids T4018–5151N | This study |
| SMC5853 | ΔvWA; SMC4798 with an unmarked deletion of LapA amino acids N4736–4963S | This study |
| SMC5851 | ΔRTX; SMC4798 with an unmarked deletion of LapA amino acids G5020–5144W | This study |
| SMC5855 | ΔT1SS; SMC4798 with an unmarked deletion of LapA amino acids N5151–5218S | This study |
| SMC5917 | lapA AA108–109RR; SMC4798 with point mutation AA108–109RR | 27 |
| SMC5915 | Pf0-1 lapG::pKO3-Tetr; SMC4798 with a single-crossover knockout mutation disrupting the lapG gene | 27 |
| SMC5927 | lapA A108W; SMC4798 with point mutation A108D | This study |
| SMC5923 | lapA AA108–109DD; SMC4798 with point mutation AA108–109DD | This study |
| SMC5925 | lapA A109D; SMC4798 with point mutation A109D | This study |
| SMC6400 | Pf0-1 lapG::pKO3-Tetr; SMC6370 with a single-crossover knockout mutation disrupting the lapA gene | 30 |
| SMC5869 | lapA ΔA108–120A; SMC4798 with an unmarked deletion of lapD and an unmarked deletion of LapA amino acids A108–120A | This study |