TABLE 2.
Fold changes in tbp expression in the Ω cassette insertion mutants compared to the wild-type strain determined by qRT-PCR
| Strain |
porBIA normalization ofa: |
|
|---|---|---|
| tbpB | tbpA | |
| WT/MCV113 | 6.30 (4.20–9.58) | 6.07 (3.94–9.32) |
| WT/MCV114 | 1.98 (1.07–3.20) | 1.86 (1.04–2.48) |
a
Numbers in boldface are the means from three experimental replicates from each of three biological replicates conducted on different days (total of nine samples). Numbers in parentheses indicate the range of values obtained by analysis with the 2−ΔΔCT method (34, 35). All fold differences comparing the wild-type strain to the mutants for both tbpB and tbpA were statistically significant (P < 0.05).