TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) | Purpose |
|---|---|---|
| JW102 | GCGGTGACCCGGGAGATCTGAATTC | ChIP-chip |
| LlacZ1291F | CGGTAGAATTCGAGCTCGGTACCT | EMSA |
| Cy3-llacZ-Ra | ACGTTGTAAAACGACGGGATC | EMSA |
| gnd-F | CCACCGACGCAGTCATCA | qRT-PCR |
| gnd-R | TGATGTCGCCTTCGTCCAT | qRT-PCR |
| 16S rRNA-F | TCGATGCAACGCGAAGAAC | qRT-PCR |
| 16S rRNA-R | GAACCGACCACAAGGGAAAAC | qRT-PCR |
| EcoRV-icd–400F | CGCGATATCGCCTCCATCACTGTGAAGTG | Promoter-lacZ construction |
| EcoRV-icd-15R | CGCGATATCAATGATCTTGGCCATGAGTCTC | Promoter-lacZ construction |
| EcoRV-gapB–400F | CGCGATATCGGTGTCCTCTCGCGCA | Promoter-lacZ construction |
| EcoRV-gapB-15R | CGCGATATCGATCAACGGCACCATGTCCTC | Promoter-lacZ construction |
| EcoRV-pyk–390F | CGCGATATCGTCTCCGCAGTAGTGTTTG | Promoter-lacZ construction |
| EcoRV-pyk-15R | CGCGATATCTCTATCCACGCCCATAAGCCT | Promoter-lacZ construction |
| EcoRV-maeB–410F | CGCGATATCAAGTGGGTATCGCGTCTGG | Promoter-lacZ construction |
| EcoRV-maeB-15R | CGCGATATCCAGGTCGATGGTCATATCATTTAGC | Promoter-lacZ construction |
| BglII-icd–246R | CGCAGATCTTCTTTTCTCCCGTGGAAGAGTAC | Promoter-lacZ construction |
| BglII-icd–240F | CGCAGATCTCAAGTCGAGCTCGCGG | Promoter-lacZ construction |
| BglII-gapB–289R | CGCAGATCTCATTGGGTTGAGATATGGGTAC | Promoter-lacZ construction |
| BglII-gapB–275F | CGCAGATCTTTGCGTAAGGCGGCAG | Promoter-lacZ construction |
| BglII-pyk–40R | CGCAGATCTCATTAATTCTCGATGAATCTTGC | Promoter-lacZ construction |
| BglII-pyk–34F | CGCAGATCTCCTGTGGCTTGAGGGGGA | Promoter-lacZ construction |
| EcoRV-maeB-mut GntR1-site2R | CGCGATATCCAGGTCGATGGTCATTAAGCGTAGCCTTGTTAATCGGTG | Promoter-lacZ construction |
| BglII-maeB–205R | CGCAGATCTCTTTTGGGTGGTTTTGGGGT | Promoter-lacZ construction |
| BglII-maeB–194F | CGCAGATCTCAAACAATTAAATTCATCACAAAACAC | Promoter-lacZ construction |
| LlacZLR-4354F | ATAACCGGGCAGGGGTCTAG | Promoter-lacZ construction |
| Ind7insert-checkR | GCGTCACGAACAACAGACAGC | Promoter-lacZ construction |
| LlacZLR-6425R | CGACGGCCAGTGCCAAGC | Promoter-lacZ construction |
| Ind7insert-checkF | CGAGACTGGAATTGAGGCTC | Promoter-lacZ construction |
| NheI-2434–1500–F5 | GCGGCTAGCGTGCACGGATTCCATGAAC | gntR1-FLAG construction |
| SalI-2434–1500-R4 | GCGGTCGACAAAGCCGAGGAAACAAGAGCAGG | gntR1-FLAG construction |
| C_gntR_FLAG-tag_nheI | CGCGCTAGCTTACTTGTCATCGTCATCCTTGTAGTCTGCTGCTCCGTTCAGCGTGCCCAGC | gntR1-FLAG construction |
| C_nheI_gnt3′_ver4 | GCGGCTAGCCGATATCGCTAAGAGCTTCAACGA | gntR1-FLAG construction |
a
Cy3 labeled at the 5′ end.