TABLE 1.
Residue-by-residue comparison of HSQC 1H and 13C chemical shifts for S. pneumoniae CPS10A, CPS39, CPS47F, and CPS34 and S. oralis RPS4Gna
| Polysaccharide | Residue | Structure | Chemical shifts (ppm)b |
|||||
|---|---|---|---|---|---|---|---|---|
| H-1 C-1 | H-2 C-2 | H-3 C-3 | H-4 C-4 | H-5 C-5 | H-6 C-6 | |||
| CPS10A | A | 5-β-Galf | 5.225 110.17 | 4.215 82.73 | 4.189 77.30 | 4.210 82.67 | 4.378 75.77 | 3.784, 3.862c 62.82 |
| CPS39 | 6-β-Galf | 5.223 110.17 | 4.216 82.23 | 4.096 77.46 | 4.099 83.37 | 3.981 70.22 | 3.966 67.22 | |
| CPS39deOAcd | 6-β-Galf | 5.227 110.12 | 4.216 82.22 | 4.098 77.48 | 4.099 83.34 | 3.989 70.15 | 3.953, 3.983 67.22 | |
| RPS4Gn | 6-β-Galf | 5.218 110.05 | 4.208 82.26 | 4.101 77.46 | 4.108 83.36 | 3.990 70.10 | 3.970 67.18 | |
| CPS47Fd | 6-β-Galf | 5.301 110.04 | 4.359 79.61 | 4.88 79.75 | 4.498 81.14 | 5.385 71.84 | 4.051 64.40 | |
| CPS47FdeOAc | 6-β-Galf | 5.217 110.08 | 4.210 82.26 | 4.096 77.47 | 4.102 83.40 | 3.983 70.16 | 3.950, 3.981 67.19 | |
| CPS34 | 3-β-Galf | 5.302 109.15 | 4.354 81.02 | 4.473 81.58 | 4.301 83.94 | 3.947 71.29 | 3.699 63.80 | |
| CPS10A | B | 3-β-Galp | 4.760 104.41 | 3.692 71.73 | 3.692 81.8 | 4.085 69.84 | 3.656 76.06 | 3.762 62.24 |
| CPS39 | 3-β-Galp | 4.764 103.94 | 3.683 71.28 | 3.684 81.15 | 4.082 69.41 | 3.669 75.80 | 3.768, 3.788 61.81 | |
| CPS39deOAc | 3-β-Galp | 4.767 104.00 | 3.676 71.30 | 3.704 81.03 | 4.070 69.45 | 3.666 75.76 | 3.769 61.84 | |
| RPS4Gn | 3-β-Galp | 4.507 103.91 | 3.675 70.84 | 3.742 81.09 | 4.107 69.38 | 3.730 75.96 | 3.78 61.85 | |
| CPS47F | 3-β-Galp | 4.506 103.90 | 3.666 70.78 | 3.761 80.90 | 4.083 69.25 | 3.718 76.08 | 3.777 61.85 | |
| CPS47FdeOAc | 3-β-Galp | 4.504 103.87 | 3.668 70.86 | 3.737 81.10 | 4.101 69.37 | 3.724 75.95 | 3.769, 3.788 61.85 | |
| CPS34 | 3-α-Glcp | 5.093 98.56 | 3.724 72.03 | 3.859 79.60 | 3.484 68.64 | 3.807 73.19 | 3.79, 3.88 61.48 | |
| CPS10A | C | β-Galf | 5.073 110.52 | 4.078 82.53 | 4.055 77.92 | 4.068 84.68 | 3.841 72.02 | 3.684 63.93 |
| CPS39 | β-Galf | 5.062 110.06 | 4.069 81.93 | 4.064 77.07 | 4.048 84.01 | 4.036 69.01 | 4.220, 4.240 66.79 | |
| CPS39deOAc | β-Galf | 5.056 110.06 | 4.073 82.09 | 4.055 77.23 | 4.068 83.77 | 3.834 71.32 | 3.676 63.28 | |
| RPS4Gn | 6-β-Galf | 5.071 108.62 | 4.068 81.81 | 4.100 77.51 | 4.006 83.96 | 4.027 70.78 | 3.767, 4.077 71.91 | |
| CPS47F | 6-β-Galf | 5.386 107.89 | 5.061 84.78 | 4.239 76.56 | 4.151 84.78 | 4.049 70.64 | 3.780, 4.069 71.78 | |
| CPS47FdeOAc | 6-β-Galf | 5.231 109.94 | 4.207 82.24 | 4.077 77.65 | 4.069 83.90 | 4.022 70.71 | 3.771, 4.074 71.84 | |
| CPS34 | 2-β-Galf | 5.362 108.34 | 4.271 87.35 | 4.270 76.06 | 4.103 82.55 | 4.082 68.61 | 4.247 66.46 | |
| CPS10A | D | 3,4,6-β-GalNAc | 4.788 103.76 | 4.145 53.54 | 3.928 79.29 | 4.269 76.51 | 3.938 74.84 | 3.979, 4.099 71.22 |
| CPS39 | 3,4,6-β-GalNAc | 4.747 103.60 | 4.162 52.97 | 3.896 79.04 | 4.279 75.78 | 3.955 74.37 | 3.992, 4.097 70.98 | |
| CPS39deOAc | 3,4,6-β-GalNAc | 4.74 103.61 | 4.159 52.98 | 3.894 78.94 | 4.286 75.98 | 3.958 74.32 | 3.997, 4.096 70.93 | |
| RPS4Gn | 6-β-GalNAc | 4.635 103.93 | 3.944 53.36 | 3.752 71.56 | 3.945 68.59 | 3.829 74.54 | 3.775, 3.911 68.08 | |
| CPS10A | E | 3-α-Galp | 5.203 100.36 | 3.938 68.88 | 4.006 80.70 | 4.272 70.29 | 4.057 72.25 | 3.762 62.24 |
| CPS39 | 3-α-Galp | 4.962 99.93 | 3.898 68.30 | 4.005 80.38 | 4.263 70.01 | 3.997 71.49 | 3.744 62.01 | |
| CPS39deOAc | 3-α-Galp | 4.964 99.92 | 3.906 68.28 | 4.004 80.38 | 4.266 70.00 | 3.996 71.50 | 3.745 62.00 | |
| RPS4Gn | 3-α-Galp | 4.959 99.92 | 3.890 68.26 | 3.963 80.22 | 4.204 70.08 | 3.980 71.43 | 3.74 62.06 | |
| CPS47F | 3-α-Galp | 5.247 100.03 | 3.965 68.49 | 4.016 77.92 | 4.164 69.97 | 4.083 72.03 | 3.744 61.80 | |
| CPS47FdeOAc | 3-α-Galp | 5.25 99.95 | 3.962 68.50 | 3.977 78.17 | 4.168 69.98 | 4.082 72.02 | 3.733, 3.754 61.84 | |
| CPS34 | 3-α-Galp | 5.238 100.31 | 3.957 68.52 | 4.008 77.92 | 4.143 70.28 | 4.091 72.00 | 3.747 61.80 | |
| CPS10A | F | 2-Ribitol-5 | 3.856, 3.931 61.36 | 4.017 80.81 | 3.879 71.91 | 3.998 73.18 | 4.022, 4.145 68.33 | |
| CPS39 | 1-Ribitol-5 | 3.596, 3.965 69.42 | 4.066 71.66 | 3.828 72.22 | 3.928 71.64 | 3.989, 4.080 67.39 | ||
| CPS39deOAc | 1-Ribitol-5 | 3.601, 3.968 69.41 | 4.068 71.65 | 3.829 72.21 | 3.93 71.62 | 3.994, 4.076 67.37 | ||
| RPS4Gn | 1-Ribitol-5 | 3.599, 3.965 69.48 | 4.054 71.63 | 3.818 72.29 | 3.933 71.66 | 3.99, 4.085 67.40 | ||
| CPS47F | 2-Ribitol-5 | 3.845, 3.926 60.83 | 4.052 80.62 | 3.845 71.21 | 4.023 72.39 | 3.943, 4.057 67.83 | ||
| CPS47FdeOAc | 2-Ribitol-5 | 3.840, 3.928 60.80 | 4.052 80.57 | 3.859 71.23 | 4.031 72.38 | 3.977, 4.084 67.75 | ||
| CPS34 | 2-Ribitol-5 | 3.847, 3.922 60.77 | 4.047 80.80 | 3.869 71.24 | 4.044 72.35 | 4.008, 4.096 67.79 | ||
| CPS10A | G | β-Galp | 4.435 104.84 | 3.532 72.09 | 3.627 74.23 | 3.943 69.94 | 3.676 76.30 | 3.762 62.24 |
| CPS39 | β-Galp | 4.436 104.51 | 3.511 71.56 | 3.628 73.70 | 3.921 69.41 | 3.680 75.92 | 3.782 61.82 | |
| CPS39deOAc | β-Galp | 4.439 104.47 | 3.510 71.54 | 3.631 73.67 | 3.923 69.41 | 3.685 75.91 | 3.768, 3.784 61.84 | |