FIG 4.

Crl conserved residue R51 is necessary for activation of transcription in vivo. (A) osmY promoter-lacZ expression in early stationary phase in E. coli strains with different chromosomal crl alleles. Fold activation (with or without crl) is shown above the bar for each allele. The error bars show SD for 3 cultures each with 3 replicates each. (B) Western analysis of crl WT and mutant strains. A representative gel is shown. The graph shows the ratios of Crl variants compared to the WT for 3 experiments.