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Crl conserved residue R51 is necessary for activation of transcription in vitro. (A) Multiple-round in vitro transcription from a plasmid-borne osmY promoter (pRLG8941) by EσS in the presence of WT Crl and the R51K variant in vitro. A representative experiment is shown. (B) Quantitation of activation by WT Crl and Crl variants over a range of Crl concentrations.
