FIG 5.

Effects of the nfs cluster on siderophore production in Pseudomonas sp. HYS. (A) Representation of the nfs cluster. The arrows and their direction indicate the locations and direction of transcription of predicted genes, respectively. (B) Siderophore production in wild-type HYS and the nfs mutants was detected on CAS agar plates. (C) Siderophore production in wild-type HYS and the nfs mutants in liquid MKB medium was determined as siderophore units (percent) by the CAS liquid assay. (D) Absorption spectra of filtered supernatants of 24-h MKB cultures of wild-type HYS and the nfs mutants. The error bars indicate standard deviations.