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RT-PCR of RNA extracted from Pseudomonas sp. HYS with ORF connecting primer pairs (orf1-orf2RT-1 plus orf1-orf2RT-2, orf2-orf3RT-1 plus orf2-orf3RT-2, and orf3-orf4RT-1 plus orf3-orf4RT-2). The different lanes represent different templates: lanes 1, cDNA; lanes 2, genomic DNA as a positive control; lanes 3, RNA without reverse transcription as a negative control; lanes 4, ddH2O as a blank control.
