TABLE 2.
Characteristics of the 14 transposon mutants of HYS
| Mutanta | Insertion site | Insertion gene functionb | Relative siderophore productionc |
|
|---|---|---|---|---|
| CAS plate | Liquid medium | |||
| M32 | Scaffold1 91950–91951 | Pyruvate decarboxylase | 0.31 ± 0.01 | 0.10 ± 0.00 |
| M58 | Scaffold1 93834–93835 | Phenylacetate-CoA ligase | 0.20 ± 0.00 | 0.16 ± 0.01 |
| M30 | Scaffold1 94178–94179 | Phenylacetate-CoA ligase | 0.16 ± 0.01 | 0.21 ± 0.00 |
| M38 | Scaffold1 94422–94423 | Phenylacetate-CoA ligase | 0.17 ± 0.00 | 0.17 ± 0.01 |
| M16 | Scaffold1 94637–94638 | Phenylacetate-CoA ligase | 0.15 ± 0.01 | 0.17 ± 0.00 |
| M52 | Scaffold1 94637–94638 | Phenylacetate-CoA ligase | 0.14 ± 0.01 | 0.16 ± 0.00 |
| M12 | Scaffold1 95250–95251 | Acyl-CoA dehydrogenase | 0.17 ± 0.00 | 0.17 ± 0.01 |
| M55 | Scaffold1 95252–95253 | Acyl-CoA dehydrogenase | 0.17 ± 0.00 | 0.16 ± 0.01 |
| M48 | Scaffold1 95451–95452 | Acyl-CoA dehydrogenase | 0.16 ± 0.02 | 0.12 ± 0.00 |
| M5 | Scaffold1 96196–96197 | Thioesterase | 0.37 ± 0.01 | 0.17 ± 0.01 |
| M13 | Scaffold1 96357–96358 | Thioesterase | 0.36 ± 0.00 | 0.16 ± 0.00 |
| M2 | Scaffold4 115281–115282 | GacA | 0.02 ± 0.00 | 0.17 ± 0.01 |
| M35 | Scaffold33 33826–33827 | GacS | 0.02 ± 0.00 | 0.16 ± 0.00 |
| M11 | Scaffold33 33887–33888 | GacS | 0.02 ± 0.00 | 0.13 ± 0.00 |
a
The mutants are arranged according to their relative positions in the genome.
b
Gene designation and proposed functions are based on the annotated genome sequence from Pseudomonas sp. HYS.
c
Relative siderophore production is expressed as the ratio of mutants to the wild-type HYS. On CAS plates, it was determined as the semidiameter of chelated halos; in liquid medium, it was determined as siderophore units (%) and normalized to the OD600 of the bacterial culture in MKB medium (see Materials and Methods for details). Each value is the average of three different cultures ± standard deviation.