FIG 2.
Partial loss-of-function cdsA mutants and pgsA444 suppress the vancomycin sensitivity of lptD mutants. (A) Efficiency of plating assay (EOP) on LB agar plates supplemented with 70 mg/liter, 140 mg/liter, or 150 mg/liter vancomycin and grown at 37°C overnight. Suppression is shown for lptDI54E. Note that the chromosomal lptD allele is ΔlptD, while the lptD allele listed for each EOP is present in pET2342::lptD, which has a leaky T7 promoter. T7 RNA polymerase is not present in these strains. (B) EOPs as described for panel A. Suppression is shown for lptDΔD57ΔK60.