TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant features | Reference and/or source |
|---|---|---|
| Strains | ||
| MC4100 | F− araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR thi | 17 |
| NR754 | MC4100 Ara+ | 18 |
| HC334 | NR754 ΔlptD pET2342::lptDI54E | 21, 48, 49; this study |
| HC338 | NR754 ΔlptD pET2342::lptDV52E-I54E | 21, 48, 49; this study |
| HC340 | NR754 ΔlptD pET2342::lptD | 21, 48, 49; this study |
| HC342 | NR754 cdsA7 dgt::Tn10 | This study |
| HC351 | HC334 ΔclsA::kan | 34; this study |
| HC386 | HC334 cdsA7 dgt::Tn10 | This study |
| HC509 | NR754 ΔlptD pET2342::lptDΔD57ΔK60 | 21, 48, 49; this study |
| HC510 | HC334 pgsA444 zed-3069::Tn10 | 39, 50, 51; this study |
| HC530 | HC509 cdsA7 dgt::Tn10 | This study |
| HC571 | NR754 ΔclsA::kan | 34; this study |
| HC575 | NR754 pgsA444 zed-3069::Tn10 | 39, 50, 51; this study |
| HC630 | HC509 pgsA444 zed-3069::Tn10 | 39, 50, 51; this study |
| HC960 | HC334 cdsA9 dgt::Tn10 | This study |
| HC961 | HC509 cdsA9 dgt::Tn10 | This study |
| HC962 | NR754 cdsA9 dgt::Tn10 | This study |
| HC1045 | NR754 pASKA::plsC | 35; this study |
| HC1198 | JAS193 cdsA7 dgt::Tn10 | This study |
| HC1199 | JAS193 cdsA9 dgt::Tn10 | This study |
| HC1240 | HC342 pASKA::cdsA | 35; this study |
| HC1241 | HC962 pASKA::cdsA | 35; this study |
| JAS193 | MC4100 ΔbamE::kan | J. Schwalm, unpublished data |
| Plasmids | ||
| pET2342::lptD | lptD cloned into pET2342 | 48 |
| pASKA::cdsA | IPTG inducible, Camr | 35 |
| pASKA::plsC | IPTG inducible, Camr | 35 |