FIG 4.
SDS-PAGE of methylene-H4F reductase partially purified from M. thermoacetica. Lane 1, molecular mass markers; lane 2, partially purified methylen-H4F reductase complex. The proteins were identified by MALDI-TOF MS analysis of the protein bands after digestion with trypsin. The molecular masses were deduced from the amino acid sequences. The five other proteins identified after digestion were as follows: pyruvate:ferredoxin oxidoreductase (POR) (Moth_0064), copper amine-oxidase-like (amine oxidase) (Moth_2343), GroEL (a molecular chaperonin) (Moth_0546), ATP-dependent protease (Moth_1030), and LacI/GalR, transcriptional regulator (Moth_2022). (See the text for details.)