TABLE 2.
Calcineurin is required for drug resistance in C. tropicalis
| Strain | MIC or MIC range (μg/ml)a |
||||
|---|---|---|---|---|---|
| Caspofungin | Ketoconazole | Voriconazole | Fluconazole | Amphotericin B | |
| MYA3404 (wild type) | 0.032 | 0.064 | 0.125 | 2.0 | 0.38–0.5 |
| YC454 cnb1/cnb1 | 0.016 | 0.016 | 0.047 | 1.0 | 0.38–0.5 |
| YC466 cnb1/cnb1 | 0.016 | 0.016 | 0.047 | 1.0 | 0.38–0.5 |
| YC494 crz1/crz1 | 0.064 | 0.094 | 0.19–0.25 | 3.0 | 0.25 |
| YC499 crz1/crz1 | 0.064 | 0.094 | 0.19–0.25 | 2.0–3.0 | 0.25 |
a
Cells were grown overnight at 30°C and washed twice with ddH2O. Then, cells at an OD of 0.5 (in 500 μl) were spread on RPMI 1640 medium (R04067; Remel). After 20 min, the Etest strips (bioMérieux Corp.) were transferred to the surface of the medium. The MIC was read after 24 h of incubation at 35°C according to the manufacturer's instructions.