TABLE 1.
Strains used in this study
| Strain | Parental strain | Genotype |
|---|---|---|
| RIB40a | Wild type | |
| NSRKu70-1-1b | niaD− sC− adeA− ΔargB::adeA− Δku70::argB | |
| NSK-Δlah2 | NSRKu70-1-1 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA |
| NSK-Δwsc1 | NSRKu70-1-1 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAowsc::adeA |
| NSK-Δhex1-1 | NSRKu70-1-1 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAohex1::adeA |
| NSRKu70-1-1Ab | NSRKu70-1-1 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB pAdeA (adeA) |
| NSK-LAH[1–2039]G | NSRKu70-1-1A | niaD− sC− adeA− ΔargB::adeA− Δku70::argB pAdeA (adeA) pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) |
| NSK-Δlah-LAH[1–2039]G | NSK-Δlah2 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) |
| NSK-Δwsc-LAH[1–2039]G | NSK-Δwsc1 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAowsc::adeA pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) |
| NSK-LAH[4710–5727]G | NSRKu70-1-1A | niaD− sC− adeA− ΔargB::adeA− Δku70::argB pAdeA (adeA) pgAoLAH[4710–5727]G (P-amyB::Aolah[4710–5727]-egfp::T-amyB::niaD) |
| NSK-Δlah-LAH[4710–5727]G | NSK-Δlah2 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[4710–5727]G (P-amyB::Aolah[4710–5727]-egfp::T-amyB::niaD) |
| NSK-Δlah-LAH-3HA | NSK-Δlah2 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAHN (P-amyB::Aolah-3×HA::T-amyB:: niaD) |
| NSK-Δlah-LAH[1–2039]G-LAH-3HA | NSK-Δlah-LAH[1–2039]G | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) pgAoLAHN (P-amyB::Aolah-3×HA::T-amyB:: niaD) |
| NSK-LAH[(1–2039)+(4710–5727)]-3HA | NSK-Δlah2 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[(1–2039)+(4710–5727)]N (P-amyB::Aolah[(1–2039)+(4710–5727)]-3×HA::T-amyB::niaD) |
| NSK-LAH[1–2039]G-LAH[(1–2039)+(4710–5727)]-3HA | NSK-Δlah-LAH[1–2039]G | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) pgAoLAH[(1–2039)+(4710–5727)]N (P-amyB::Aolah[(1–2039)+(4710–5727)]-3×HA::T-amyB::niaD) |
| NSRKu70-1-1AN | NSRKu70-1-1A | niaD− sC− adeA− ΔargB::adeA− Δku70::argB pAdeA (adeA) pNR10 (niaD) |
| NSK-ΔlahN | NSK-Δlah2 | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pNR10 (niaD) |
| NSK-LAH[1–2039]G-DPTS1 | NSK-Δlah-LAH[1–2039]G | niaD− sC− adeA− ΔargB::adeA− Δku70::argB ΔAolah::adeA pgAoLAH[1–2039]G (P-amyB::Aolah[1–2039]-egfp::T-amyB::AosC) pgDPTS1N (P-amyB::mdsred-pts1::T-amyB::niaD) |