FIG 1.

(A) Effects of UPC2 on MICs and growth on YPD agar as determined by Etest. A confluent lawn of C. albicans was streaked prior to the addition of Etest strips and was then incubated for 48 h. (B) MIC heat map of SC5314, the UPC2 mutant, and a complemented derivative. Susceptibility was determined by broth microdilution in YPD at 72 h (MICs in µg/ml above heat map). Growth was quantified spectrophotometrically and was assigned to a colorimetric scale. (C) Effect of UPC2 on the ability to grow on a solid medium containing fluconazole. From 4-fold serial dilutions of C. albicans strains, 2-μl aliquots were spotted onto YPD agar with (right) or without (left) 10 μg/ml FLC and were incubated for 48 h. (D) Effect of fluconazole on UPC2 in a time-kill assay. SC5314 or upc2Δ/Δ cells were diluted in YPD medium containing fluconazole (10 μg/ml) or the solvent dimethyl sulfoxide (DMSO). After 0, 6, 12, and 24 h, samples from each strain and medium were diluted and were plated for CFU.