FIG 2.
Cell flow sorting of S. aureus labeled with a fluorescent reporter in cheese. CIM433 served as a negative gating control. CIM433 carrying plasmid pIF1, pCM11, or pIF2 was used for the FCM test. The cell density was adjusted to 106 CFU/ml after filtering, and populations of 20,000 cells were separated by FCM after molding for 4 h (a) and after 1 (b) and 15 (after core [c]-surface [d] separation) days (insets show zoom on cytographs). The percentage of fluorescent cells collected with a 527-nm bandpass filter (FL1 channel) and the fluorescence intensity of each signal are indicated.