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Transmission electron micrographs of the Clostridium botulinum ATCC 3502 parent (A) and sigF (B), sigE (C), and sigG (D) mutant strains with sense-oriented ClosTron insertions grown in TPGY broth for 7 days at 37°C. Indicated are the nucleoid area (N), spore coat (SC), spore cortex (CX), forespore membrane (FM), forespore (FS), and potential septation membranes (SM).
