FIG 2.

Flow cytometry cytograms resolving bacteria, characterized by two populations based on their DAPI fluorescence and structure, discriminating low and high nucleic acid contents (LNA and HNA, respectively) (right) and picoautotrophs (<2 μm), where three populations are distinguished by their red (630LP, chlorophyll A) and orange (580/30 nm, phycoerythrin) fluorescences and named phycoerythrin-rich picocyanobacteria (PE-CYAN), picoeukaryotes (PicoEuk), and picoorganisms (P) (left and middle), isolated from biofilms scraped from two different coatings, PVC and AS3. u.a., arbitrary units; SSC, side scatter.