TABLE 1.
Primers used in construction of pALKO1-2
| Primer namea | Sequence |
|---|---|
| PMC500BACK_F | TCTATCAGCTGTCCCTCCTGTTC |
| PMC500BACK_R | CCAGGCATCAAATAAAACGAAAG |
| 0361upstreamF | CAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCATCTTATAAGATAGTGTACGAGGAAAGTG |
| 0361KOupWT_R | GTATAATCTTACCTATCACCTCAAATGGTTCGCTGGGTTTGGAATAAATCCTCCTCCTTAATATATAGTG |
| 0361KOdownWT_F | CTAGATAGGGGTCCCGAGCGCCTACGAGGAATTTGTATCGCACAAGCCTAACAAAAAAGTCCTC |
| 0361downstreamR | CCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAGCAAGTGATCTAAATCTAAATATCTTCTTC |
| kanregion_F | AAACCCAGCGAACCATTTGAGG |
| kanregion_R | CGATACAAATTCCTCGTAGGCGC |
a
F, forward; R, reverse.