FIG 2.

Growth of Xanthomonas sp. 35Y on latex agar and staining of degradation products by Schiff reagent. (A) ΔroxA Xanthomonas sp. (left), ΔroxA Xanthomonas harboring pNH1::roxA (middle), and ΔroxA Xanthomonas harboring pNH1::lcp (right) were grown on solid latex plates (top row) for 3 weeks at room temperature and subsequently stained with Schiff reagent (bottom row). There was no fuchsin staining for RoxA (bottom middle) because of uptake and the use of degradation product ODTD. Note the clearing zone for RoxA (top middle) and stained halo for Lcp (bottom right), presumably because of insufficient uptake of Lcp-derived degradation products that are considerably larger than ODTD. (B) Activity assay for purified Lcp after staining with Schiff reagent (cuvette on the left with Lcp, cuvette on the right without Lcp).