FIG 3.

(A) Elution profile of Lcp-containing Q-Sepharose pool on hydroxyapatite. A 55-ml portion of a concentrated Lcp pool after chromatography on Q-Sepharose was applied to a 20-ml hydroxyapatite column that had been equilibrated with 1 mM potassium phosphate buffer (pH 6) and eluted with a 1 to 200 mM potassium phosphate gradient in equilibration buffer. Solid and dotted lines represent the absorbance at 280 nm and conductivity, respectively. Note that the second sharp peak of the gradient contained Lcp (arrow) and illustrates the separation power of hydroxyapatite. (B). SDS-PAGE of Lcp-containing fractions at different stages of purification. The gel was stained with silver. Molecular mass markers are indicated. HyAp, hydroxyapatite step; QFF, Q-Sepharose Fast Flow step.