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pH profile of Lcp-catalyzed cleavage of polyisoprene. Activity assays were performed as described in Materials and Methods using different buffer systems: 50 mM acetate buffer (●), 0.1 M potassium phosphate buffer (□), 50 mM HEPES buffer (○), 0.1 M Tris-HCl (◼), or 0.1 M carbonate buffer (▲). The area of the major product peak after HPLC analysis at 22.9 min is shown.
